i-Biology Patent

Patent No.:

9,649,303

Date of Patent:

Mar. 31, 2015

What is claimed is:

1. A recombinant DNA construct comprising a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 operably linked to at least one heterologous sequence, wherein said nucleotide sequence is a promoter.

2. The recombinant DNA construct of claim 1, wherein the heterologous nucleotide sequence encodes a gene involved in anthocyanin biosynthesis, a gene involved in the synthesis of fragrant fatty acid derivatives, a gene that is determinative of flower morphology, or a gene involved in biosynthesis of plant cytokinin.

3. The recombinant DNA construct of claim 2, wherein the gene involved in anthocyanin biosynthesis is dyhydroflavonol 4-reductase, flavonoid 3,5-hydroxylase, chalcone synthase, chalcone isomerase, flavonoid 3-hydroxylase, anthocyanin synthase, or UDP-glucose 3-O-flavonoid glucosyl transferase.

4. The recombinant DNA construct of claim 2, wherein the gene involved in the synthesis of fragrant fatty acid derivatives is S-linalool synthase, acetyl CoA:benzylalcohol acetyltransferase, benzyl CoA:benzylalcohol benzoyl transferase, S-adenosyl-L-methionine:benzoic acid carboxyl methyl transferase, mycrene synthase, (E)-.beta.-ocimene synthase, orcinol O-methyltransferase, or limonene synthase.

5. The recombinant DNA construct of claim 2, wherein the gene that is determinative of flower morphology is AGAMOUS, APETALA, or PISTILLATA.

6. The recombinant DNA construct of claim 2, wherein the gene involved in biosynthesis of plant cytokinin is isopentenyl transferase.

7. A vector comprising the recombinant DNA construct of claim 1.

8. An isolated cell comprising the recombinant DNA construct of claim 1.

9. The cell of claim 8, wherein the cell is a plant cell.

10. A transgenic plant having stably incorporated into its genome the recombinant DNA construct of claim 1.

11. The transgenic plant of claim 10, wherein the plant is a flowering plant.

12. The transgenic plant of claim 11, wherein the flowering plant is rose, carnation, Gerbera, Chrysanthemum, tulip, Gladioli, Alstroemeria, Anthurium, lisianthus, larkspur, irises, orchid, snapdragon, African violet, or azalea.

13. A transgenic seed produced by the transgenic plant of claim 12, wherein the transgenic seed comprises the recombinant DNA construct.

14. A method of expressing a coding sequence or a functional RNA in a plant comprising: a) introducing the recombinant DNA construct of claim 1 into the plant, wherein the at least one heterologous sequence comprises a coding sequence or encodes a functional RNA; b) growing the plant of step a); and, c) selecting a plant displaying expression of the coding sequence or the functional RNA of the recombinant DNA construct.

15. A method of transgenically altering a marketable flower trait of a flowering plant, comprising: a) introducing a recombinant DNA construct of claim 1 into the plant; b) growing a fertile, mature plant resulting from step a); and c) selecting a plant expressing the at least one heterologous nucleotide sequence in at least one plant tissue based on the altered marketable trait.

16. The method of claim 15 wherein the marketable flower trait is color, morphology, or fragrance.